In vitro callus induction and phytochemical screening of Corbichonia decumbens (Forssk.) Exell through GC MS analysis

Abstract

Corbichonia decumbens (Forssk.) Exell a member of Aizoaceae is an important medicinal herb. The present study aims to develop an efficient protocol for callus induction from the leaf explants of C. decumbens and assessment of phytochemical compounds in the methanolic extracts of callus. The leaf explants were cultured on MS medium supplemented with different concentrations of auxins (2,4-D and NAA) alone or in combination with BAP. Among the tested, higher frequency (96.6%) of callus induction was observed on medium containing 5 µM 2,4-D plus 2 µM BAP. The well-developed callus was pulverised and extracted with methanol for GC -MS analysis. The results revealed that 31 phytochemical compounds from methanol extracts. The major compounds were 1-Pentadecene (15.35%); 1-Heptadecene (12.98%); 2-Pyrrolidinone (10.04%); 8-Octadecanone (9.75%); 1-Nonadecene (8.00%); 8-Pentadecanone (7.77%).