Effect of seed priming on biochemical changes in rice under anaerobic condition

Abstract

Present investigation was carried out to study the “Effect of seed priming on anerobic germination of rice” during wet season, 2016-17. Experiment was laid out in randomized block design with three replication and two varieties Sambha Mahsuri and Sambha Mahsuri Sub1 in cemented pond (size; 20×20×1.25 meter). Primed seeds were direct seeded and field was completely submerged/ flooded, up to 25-30 cm water depth was maintained for one month. Recommended dose of N, P and K @ 120:40:40 Kg ha-1 was reframed with time schedule (1/4 dose of N and full dose of P2O5 and K2O were applied as basal and rest N was applied in there splits i.e. 30, 60 and 90 DAS respectively). Treatments comprised of (T1) Seed priming with GA3 @ 25 ppm, (T2) Seed priming with GA3 @ 50 ppm, (T3) Seed priming with JLE @ 2%, (T4) Seed priming with KNO3@ 0.5%, (T5) Seed priming with KCl @ 0.2%, (T6) Seed priming with NaCl @ 0. 5%, (T7) Seed priming with IAA @ 0.2%, (T8) Seed priming with CaCl2 @ 0.1%, (T9) Control with distilled water. Results indicated that all the priming treatments increased the biochemical parameters like total sugar content, reducing and non reducing sugar, protein content and increases days to effect of GA3 @ 50 ppm was found more pronounced followed by GA3 @ 25 ppm on various parameters in both the variety but Sambha Mahsuri Sub1 showed more response of seed priming as compared to that of Sambha Mahsuri.Seed priming with various chemicals and its concentration may be used as a tool to mitigate the adverse effect caused by anaerobic condition in rice or direct seeded rice early flooding in recurrent phenomena.