In vitro evaluation of fungicides against Fusarium oxysporum f. sp. Wilt of tomato

Abstract

The present study permitted “In vitro Evaluation of Fungicides against Fusarium oxysporum f. sp. lycopersici. (Wilt of Tomato).” Were conducted at the laboratory. Tomato (Lycopersicon esculentum Mill) is most important crop grown in India wilt disease is caused by Fusarium oxysporum were serious threat to crop production. The fungus Fusarium oxysporum f. sp. Lycopersici were evaluated with the six fungicides to check the in vitro evaluation against the pathogen. The six fungicides (systemic + non systemic + combi fungicides) were Cabriotop, Chlorothalonil, Custodia, Difenoconazole, Azoxistrobin and Azoxistrobin + Difenoconazole. Three replications were conductedfor each fungicide concentration (50, 100, 150ppm). Desired concentrations obtained by adding appropriate amount of oil to PDA in petri plates by Poison food technique. Average mycelial growth was reported with the evaluation of fungicides at 50, 100, 150 ppm ranged between 29.11mm to (Custodia) 47.33mm (Azoxistrobin + difenoconazole). Maximum average radial mycelial growth was recorded with the treatment, Chlorothalonil (37.88mm), Difenoconazole (40.66mm), Cabriotop (42.55mm), Azoxystrobin (43.88mm), Azoxistrobin + difenoconazole (47.33mm). Thus, all the fungicides against Fusarium oxysporum f. sp. lycopersici and inhibited its mycelial growth over untreated control. Fungicides found most effective in the order of merit were Custodia, Chlorothalonil, Difenoconazole, Cabriotop, Azoxystrobin, Azoxystrobin + difenoconazole.