In ovo antiviral activity of Andrographis paniculata against NDV2K35 strain of Newcastle disease virus

Abstract

The antiviral activity of Andrographis paniculata was assessed in aqueous extract, ethanolic extract, andrographolide isolated from ethanolic extract and andrographolide standard against NDV2K35 strain of Newcastle disease virus by in ovo technique. The results were compared with standard antiviral drug ribavirin. All the embryo survived in treatment groups during the experiment. Haemagglutination was absent in all the groups except virus control and in groups treated with ethanolic extract. The absence of virus in these groups was further confirmed by RT-PCR for F gene. Thus, the present study confirms the antiviral activity for aqueous extract of Andrographis paniculata, andrographolide isolated from ethanolic extract and andrographolide standard against NDV2K35 strain of Newcastle disease virus.