Incorporation of the high grain protein gene Gpc-B1 (Zn and Fe) in high yielding wheat cultivar through marker assisted backcross breeding

Abstract

Grain protein content in wheat has been a major trait of interest for breeders since it has enormous end use potential. Marker-assisted backcrossing (MABC) was successfully uses to improve GPC in wheat cultivar. The genotype high Gpc-B1 donor parent use for incorporation of the gene Gpc-B1 that confer high GPC. For segregating population, SSR marker Xucw108 generally use foreground selection to select plants carrying Gpc-B1. For background selection generally 86 polymorphic SSR marker use for to recover the recipient parent genome. Eliminating linkage drag, marker spanning 10 cM region around the gene Gpc-B1 were employed to select lines with a donor segment of the minimum size carrying the gene of interest. They improves lines have significantly higher GPC and near about 88.4 to 92.3 percent of recurrent parent genome recover generally. The whole exercise of transfer of Gpc-B1 and reconstitution of the genome were generally complete within period of two and half years (five crop cycle) demonstrating practical utility of MABC for developing high GPC lines in the background of any elite popular wheat cultivar.