Serodiagnosis of Tobacco streak ilarvirus infecting peanut (Arachis hypogea) in villages of Chittoor district, Andhra Pradesh

Abstract

Tobacco streak virus (TSV) is most devastating plant pathogen; causing severe impact on the crop production and productivity. TSV is distributed throughout the globe; infecting species under families of the dicots and monocots. Epidemics of the TSV on the peanut and sunflower occur in India. In the present study, we have collected leaf and stem samples from the peanut plant showing chlorotic and necrotic lesions from the fields of Gajulamandyam, Tirupathi and chandragiri villages of Chittoor district. Collected samples were serodiagnosed by direct antigen coating (DAC)-ELISA using TSV antisera. Twenty six field samples were found positive towards the TSV antisera out of 165. The ELISA positive samples were further confirmed through Dot immunobinding assay (DIBA). The remaining 139 field samples were found negative for against TSV antisera; may be infected other plant pathogens or physiological conditions