HPTLC quantification of triterpenoid compounds and evaluation of in-vitro potential of Trichodesma indicum L

Abstract

Trichodesma indicum (Linn.) R.Br. is traditionally used folk medicine, having a lot of pharmacological bustles. The current study is focused on investigating the phytochemical and biological evaluation of T. indicum. Quantification of oleanolic acid, lupeol, and linolic acid is done by using a tertiary mobile phase consisting of toluene: ethyl acetate: formic acid (7: 2.5: 0.5 v/v). Oleanolic acid, lupeol, and linolic acid were resolved at Rf 0.46 ± 0.04, 0.55 ± 0.04 and 0.62 ± 0.05 respectively and it was quantified at its wavelength of maximum absorbance of 600nm after derivatization with anisaldehyde sulphuric acid reagent. The limits of detection and limit of quantification were found to be 662.4, 665.18 and 660.26ng per ml and 2007.4, 2015.7 and 2000.78 ng per ml for linolic acid lupeol, and oleanolic acid, respectively. The linear regression data for the calibration plot showed a good linear relationship between peak area and concentration in the range of 200-600ng/Spot. The developed HPTLC method is accurate, precise and has been successfully applied for the assay of this bioactive molecule in Trichodesma indicum. The pharmacological potential of the extract is dependent on the contents of active secondary metabolites present in the plants. The antioxidant potential was studied by using DPPH and 2-deoxyribose assay. Antioxidant potential was observed showing IC50 values (326.82±1.21µg/ml) and (449.62±2.01µg/ml) by DPPH and 2-deoxyribose method respectively. In vitro antidiabetic potential of the plant were also assessed by starch iodine assay and DNS method. IC50 was observed (86.57 ± 2.57µg/ml) in 3, 5 DNS assay and (222.86 ± 3.36 mg/ml) in starch iodine assay. Results are promising; this work forms a firm base for further research to explore the lead compound which is responsible for the medicinal value of this plant.