Bio-virus inhibitors with potential for retrieval of virus free plants of Lilium spp

Abstract

The anti-viral property of plant extract derived from medicinal plants viz., Asparagus, Centella, Tinospora and Vitex have shown tremendous virus inhibitory potential for production of virus free plants of Lilium both under field conditions and in-vitro conditions. The extracts of these plants were prepared in different solvents using soxhlet apparatus. Serological indexing was done before applying the extract as well as after applying the extract. Only those samples were considered infected where A405nm value exceeded two times the respective healthy control. Under in-vitro conditions, the extract was added at two levels: one was to add extracts to standardized tissue culture medium before autoclaving other was after autoclaving. Asparagus extract in acetone (AA) appears to exhibit maximum inhibitory potential at 10 mg/l, when added to medium after autoclaving. However, inhibition potential was lost when added before autoclaving. Centella and Tinospora extracts in acetone (CA) and water (TW), respectively inhibited LSV significantly. Field sprays on the other hand could not eliminate virus under test. However, inhibition to certain degree was recorded.