Genetic diversity assessment of Sinarundinaria anceps (Mitf.) Chao and Renvoize accessions through Isozyme markers

Abstract

Hill bamboo (Ringal) is socio-economically and ecologically important bamboo in Himalayan range. Sinarundinaria anceps have wide variety of uses ranging from household to commercial purposes. Due to over use of the species, serious deterioration of natural resources have been recorded. In light of these facts, as attempt has been made to assess genetic diversity through isozyme marker with four enzyme system (peroxidase, esterase, malate dehydrogenase and malic enzyme) prevailed in the genotypes of S. anceps collected from different localities of Garhwal Himalayas (India) and established at Hill bamboo Germplasm at Khirsu (India). Native PAGE was performed for the resolution of isozymes and bands were scored as binary data (0 or 1). Data obtained was analyzed under cluster analyses by using Jaccard´s similarity coefficient and UPGMA method. Very high degree of similarity was reported i.e. 54 to 100% among different accessions. Dendrogram constructed revealed two major cluster with nine accessions in first cluster and one accession in second cluster. First cluster was further divided in three minor clusters. The results recorded reflected very low degree of genetic diversity which necessitates in situ conservation of the natural genetic resources of the species.