Optimization of β-Glucosidase assay and protein estimation from various parts of Rauvolfia tetraphylla

Abstract

Rauvolfia tetraphylla is one of the medicinally most important plant. b-glucosidase enzyme was extracted from different plant parts of Rauvolfia tetraphylla, its crude enzyme activity was found highest in very young leaf (100%) followed by mature leaf-1 (87.16%) and mature leaf -2 (92.16%) whereas lowest in old leaf (15.0%). It was also noticed that flowers of Rauvolfia tetraphylla have highest soluble protein content in mature leaf–1 (9.241 mg/ml) which was followed by very young leaf (9.216 mg/ml). Rauvolfia tetraphylla have maximum 3 isoforms were found in flower steam, very young and mature -1 leaves.