Screening and characterization of bacteriocin producing lactic acid bacteria as probiotic from cow and buffalo milk

Abstract

Bacteriocins are natural peptides secreted by many varieties of bacteria for the purpose of killing other bacteria. This provides them with a competitive advantage in their environment, eliminating competitors to gain resources. In this study, a total of 51 bacterial strains were isolated from the 65 milk samples different regions, Dharmapuri district. 28 isolates were isolated from buffalo milk samples and 23 isolates were isolated from cow milk samples.C-1, B-4, B-13 and B-14, which had the highest activity to both indicators, were inoculated over it and incubated for 24 hours at 30 oC. The production of lactobacilli C-1, B-4, used in optimized in different concentration of lactose, different pH, different temperature. The weight of the protein bands was approximately 97kDa to below 14.3 kDa by using SDS Page electrophoresis.