Polymorphism in MHC Class II DYA EXON 2 and 3 of Deoni cattle

Abstract

Sixty DNA samples of Deoni cattle maintained at College of Veterinary Science, Rajendranagar were studied for polymorphism (PCR- RFLP) in Exon 2 and Exon 3 of Cattle MHC Class II (BoLA) DYA gene. The two fragments of the gene were amplified by specific cattle sequence primers and digested each with two enzymes. On digestion of exon 2 with Alu I resulted in two fragments of 179bp and 74bp whereas, Hae III produced 210bp and 43bp fragments in all samples. Exon 3 (296bp) digested with AvaII and Hinf I each produced two fragments of sizes 180bp, 116bp and 171bp, 125bp respectively in all samples indicating absence of polymorphism.