Molecular diversity in sweet sorghum genotypes (Sorghum bicolor L.) using SSR markers

Abstract

The present investigation was undertaken to study the genetic diversity in selected sweet sorghum genotypes at molecular level. The genomic DNA isolated from thirty sweet sorghum genotypes viz., CSV -313, RSSV-269, RSSV-260, RSSV-167, RSSV-350, RSSV-355, RSSV-453, RSSV-430, RSSV-417, RSSV-404, RSSV-397, RSSV-386, RSSV-494, RSSV-493, RSSV-483, RSSV-466, RSSV-454, RSSV-508, RSSV-503, RSSV-502, RSSV-498, RSSV-499, RSSV-500, RSSV-513, CSV-1955, RSSV-512, SPV-2191, RSSV-509, RSSV-495 and SSV-84 was subjected to PCR amplification using 13 SSR primers. Out of these 13 SSR primers, 10 yielded amplification and showed polymorphism. Total of 45 loci were generated by amplification with 09 polymorphic primers, of which 43 loci were polymorphic i.e 90% polymorphism. The maximum number of bands were observed in RSSV-355, RSSV-498, RSSV-499, SPV-2191(23 bands), whereas minimum number of bands were present in RSSV-500 (14 bands) and RSSV-313 (16 bands), CSV-19SS (17 bands). The Dice similarity coefficient values ranged from 0.10 to 0.95. Out of total 13 SSR primers used, 4 primers found to be more informative based on PIC values irrespective of per cent polymorphism. The Dice similarity coefficient values for SSR primers indicated that moderate diversity among sweet sorghum genotypes. The identical clustering pattern was observed by SSR primers. Unique loci produced by SSR primers may be the variety specific.