Influence of temperature, pH and nutritional sources on mycelial growth and sporulation of Pyricularia grisea

Abstract

Temperature, pH, carbon and nitrogen sources were tested against mycelia growth and sporulation of Pyricularia grisea causing blast diseases of rice. Among different temperatures ranges tested maximum mycelial growth and sporulation was observed at 250C (67.20 mm) with sporulation of 4.5x106 conidia/ml after 144 hrs of incubation. Out of five pH levels, maximum mycelial growth (39.50 mm) and sporulation (4.9x106 conidia/ml) observed at 7 pH. Among five carbon sources tested maltose supported good growth (32.25 mm) followed by dextrose (26.20 mm) after 144 hr of incubation. Among five nitrogen sources tested barium nitrate supported good growth (61.20 mm) followed by Ammonium nitrate(45.80 mm). None of carbon and nitrogen sources induced the sporulation of Pyricularia grisea.