Compounds isolation and in vitro antioxidant activity evaluation of Faidherbia albida (Del.) A. Chev. Leaves ethanolic extract

Abstract

Eight phenolic compounds were successively isolated and identified from the ethanolic extract of the leaves of Faidherbia albida. Five of them were previously isolated from the genus Acacia, such as I²-amyrin (L-1), I²-sitosterol (L-2), I²-sitosterol-3-O-I²-D-glucopyranoside.(L-3), Quercetin (L-5) and Gallic acid (L-6), the other three are firstly reported for isolation from the genus Acacia, and named Rhamnocitrin (L-4), Afzelin (L-7) and (6S, 9S)- Roseoside (L-8). The structures were identified and confirmed through different spectroscopic methods including 1H-NMR, 13C-NMR, HMBC and UV spectroscopy, in addition to comparison with authentic samples. Antioxidant activity was determined by the DPPH method revealed that all the tested extracts and fractions exhibited strong antioxidant activity especially ethyl acetate and aqueous fractions of the leaves.