In vitro propagation of Zingiber officinale through rhizome and effect of plant growth regulators

Abstract

Ginger (Zingiber Officinale Rosc.) is a perennial herb. It belongs to the family Zingiberaceae and commercially cultivated in most tropical regions of the world. In the present study, An efficient simple micro propagation method was developed for Ginger (Zingiber Officinale Rosc.) using fresh rhizome sprouting bud in semisolid culture media. Sprouting buds were sterilized and cultured onto MS medium supplemented with different concentrations and combinations of BAP (6-benzyl- amino-purine) and NAA (α- Naphthalene acetic acid) for shoot and root induction. Explants cultured on MS basal medium supplemented with 2.5 mg/l BAP + 1 gm/l NAA showed the highest rate of shoot multiplication.