Anti-bacterial evaluation of botanical formulation for the management of bacterial blight of pomegranate caused by Xanthomonasaxonopodis Pv. punicae

Abstract

Inhibitory activity of solvent extraction of botanicals Viz., Allium sativum, Garciniaindicaand Prosopisjuliflora, were evaluated against Xanthomonasaxonopodis pv. Punicae under in-vitro condition. Botanicals which are extracted from soxhletapparatus using different solvents. Among the botanicals tested Prosopisjuliflora with ethanol extract was found to be most effective with the highest average zone of inhibition of 31.33 mm. Followed by Methanol extract of Prosopisjuliflora (30.67 mm), Methanol extract of Garciniaindica (29.67 mm), methanol extracts of Allium sativum (24.67 mm). Shelf life of effective solvent botanical formulations stored at 4°C, 25°C, 30°C, 35°C and 40°C under in vitro conditions. Results reaveled that solvent extraction of Prosopisjuli flora recorded more efficiency, followed by solvent extraction of Allium sativum and Garciniaindica against Xanthomonasaxonopodis pvInhibitory activity of solvent extraction of botanicals. punicae.