In-vitro investigation of antioxidant activity of Cissus adnata in different fractions

Abstract

This study was conducted to evaluate the antioxidant property of Cissus adnata. The plant belongs to the vitaceae family and locally used for the treatment of tumors, boils and buboes. This study provides a scientific basis for the use of Cissus adnata in traditional medicine. The whole plant was extracted using methanol and different fractions were obtained using different organic solvents, e.g., dichloromethane, petroleum ether, chloroform. All these fractions were subjected to antioxidant activity determination. Among all the fractions, aqueous soluble fraction showed highest level of total phenolic content of 34.65 mg of GAE / gm of extractives comparing to the others. Whereas, for the DPPH free radical scavenging assay, petroleum ether fractions provided the highest IC50 value of 546.29 i­g / ml.