Evaluation of cardiac biotolerance from total aqueous extract of Entada mannii (Fabaceae) at the wistar rat

Abstract

For four weeks of treatment, we evaluated the cardiac bio-tolerance of Entada mannii (Fabaceae) in rats through enzymatic and metabolic bio-chemical markers. Entada mannii is a plant which leaves and barks are used in the treatment of malaria and diabetes in Cote d’Ivoire. The treated rats through oral route with different doses varying from 0 to 800 mg/kg of body weight permitted to determine the serum enzymatic activity of aspartate amino transferase (ASAT), lactate dehydrogenase (LDH), the creatinekinase isoenzyme-MB (CK-MB) as well as the serum concentrations of triglycerides, total cholesterol and HDL-cholesterol. The statistical analysis of the results shows significantly decrease (p˂0.05) in the activity of ASAT and CK-MB as well as a non-significant variation in the activity of HDL. On the other hand, it highlights very significant increases (p˂0.01) in the concentrations of triglycerides, total cholesterol and HDL-cholesterol from the end of the third week of treatment. But, the total cholesterol /HDL-cholesterol ration is lower than 4.5 whatever the doses eliminates the risk of cardiovascular accident. In conclusion, the aqueous extract of Entada mannii would be well tolerated by the body. However, to minimize eventual risk in human therapeutic, it would be judiciously to reduce dose to 400 mg/Kg and period of treatment to two week.