Comparative effects of inorganic and organic trace minerals (Zn, Se and Cr) supplementation on expression of ChTLR2b gene in broilers

Abstract

Poultry is the fastest growing livestock sector in the developing countries. The global poultry sector is expected to continue to grow, as demand for poultry meat is driven by growing needs, population, rising incomes and urbanization. The present experiment was conducted to investigate the effect of inorganic and organic trace minerals supplementation on expression of ChTLR2b gene in broilers. A total of 216 broilers randomly divided into twelve groups and each group consisting of 18 broilers in 3 replicates. T1 group was kept as control. T2, T3 and T4 group was supplemented with zinc (40 mg/kg of feed) inorganic, organic and 50% organic form respectively. T5, T6 and T7 groups was supplemented with selenium (0.3 mg/kg of feed) from inorganic, organic and 50%organic form respectively. T8, T9 and T10 groups was supplemented with chromium (2 mg/kg of feed) from inorganic, organic form and 50% organic form respectively. T11 and T12 group was supplemented with combination of all 3 minerals from inorganic and organic form respectively. RT-PCR expression analysis of ChTLR2b gene in spleen revealed that maximum up regulation (3.8413 fold) was found in T3 group (supplemented with organic Zn @ 40 mg/Kg of feed), followed by T4 (3.5325 fold) whereas in bursa of fabricius the maximum up regulation (2.8921 fold) was found in T6 group followed by T12 (2.5310 fold ) as compared to control group. Up-regulation of gene expression ChTLR2b in bursa of fabricius and spleen indicates beneficial effect of organic trace minerals in potentiation of immune system in broilers.