Optimization of culture conditions for enhanced decolorization of Amido black by Calocybe indica (cbe 1515) spent mushroom substrate

Abstract

The objective of this study was to exploit the decolorization potential of Spent Mushroom Substrate (SMS) of Calocybe indica (Strain-CBE 1515) for the biodegradation of reactive textile dye Amido black. Initial studies have been made on screening of mycelia and crude enzyme extract of spent compost of Milky mushroom (Calocybe indica) strains for their dyes (Amido black, Congo red and RBBR) decolorization potential. It was observed that strains of C. indica were found to be hyper specific to laccase production and significant decolorization potential was showed by CBE 1515 for Amido black. Various process parameters like composition of basal nutrient medium, pH, temperature, additional carbon and nitrogen sources, and initial dyestuff concentration were optimized to develop an economic decolorization process. The optimum dye decolorization was achieved in LME medium containing dextrose and yeast extract as carbon and nitrogen sources respectively and adjusting the pH to7.5 and incubated at 30 °C.