Effect of gamma rays, ethyl methane sulphonate and sodium azide on seedling traits, fertility and varietal sensitivity in Mungbean (Vigna radiata (L.) Wilczek)

Abstract

Seeds of two mungbean varieties viz., WGG-42 and LGG-460 were treated with various dose/concentrations of gamma radiation (200, 300, 400, 500 and 600 Gy) and ethyl methane sulphonate (0.2%, 0.3%, 0.4%, 0.5% and 0.6%) and sodium azide (1 mM, 2 mM and 3 mM) for studying the effect of the mutagens on seedling emergence (%), seedling survival (%), seedling height (cm), pollen fertility (%) and seed fertility (%) in M1 generation. The increasing dose/concentration of mutagens, decreased seedling emergence (%), seedling survival (%), seedling height (cm) and pollen fertility (%) in both the varieties of mungbean. The reductions in all these traits were observed to be more prominent in SA treatments followed by EMS and gamma rays treatments. The variety WGG-42 was found highly sensitive to various mutagens when compared with LGG-460.