In vitro evaluation of bio control agents against black spot of rose caused by Diplocarpon rosea

Abstract

The in vitro screening of Trichoderma species was to evaluate the inhibition and control the disease of black spot of rose caused by the pathogen Diplocarpon rosea. Which was a severe problem in rose gardens. Treatment with these biocontrol agents against the pathogen in dual culture method in in vitro conditions. We have taken three antagonistic fungi of Trichoderma species in which amongst the Trichoderma viride is effective in controlling the pathogen against D.rosea in aseptic conditions.