Molecular variability among the isolates of Trichoderma viride

Abstract

The molecular variability was studied among the six isolates of Trichoderma viride collected from different region of Maharashtra by using 16 RAPD primers of OPA and OPB series. Out of which 10 primers produced 78 scorable bands. Out of 78 bands, 76 bands were polymorphic and level of polymorphism was 97.32%. The isolate Tv2 (Pune) had higher value of similarity coefficient (0.400) with Tv5 (Sangli) whereas, Tv1 (Akola) had lower value of similarity coefficient (0.087) with Tv2 (Pune) and also with Tv4 (Amravati).