Seed mycoflora of some pulses collected from Bundelkhand region

Abstract

The present paper deals with a study to identify the fungi associated with seed of four major pulses of Bundelkhand namely Chickpea, Mung bean, Pigeon pea, and Lentil. Isolation of seed mycoflora was done by both blotter and Agar technique prescribed by ISTA 1996. Both the sterilized and unsterilized seed were taken. Further the effect of seed mycoflora on the germination percent of the seeds was estimated. In our study 13 genera and 23 species were isolated. There was variation in total CFU and number of seeds associated with these pulses. Chickpea possessed maximum number of seed fungi. As well as showed its superiority in germination percentage among the other legumes.