Analysis of molecular variation among diverse background wheat (Triticum aestivum L) genotypes with the help of ISSR markers

Abstract

Inter Simple Sequence Repeat (ISSR) provide adequate information about polymorphism and reproducible fingerprinting profiles for evaluating heritable diversity in different crops including wheat. The main objective of this study was to reveal the genetic diversity and relationships among different wheat genotypes. Genomic DNA from twenty wheat genotypes were analyzed using 25 inter simple sequence repeats (ISSR) markers. Out of 25 ISSR primers, 16 showed high level of polymorphism and 9 primers were monomorphic in nature. 1.66 bands per primer were amplified on an average. The PIC value varied from 0.048 to 0.747 for 25 primers. RP value of 25 ISSR primers ranged from 0.95-1.96 with an average of 1.57. The primer UBC 814 was reported as best one in distinguishing the 20 durum varieties. Similarity values for all the 20 genotypes ranges from 0.087 to 0.537 showing substantial differences among the wheat varieties. The 25 ISSR markers were used to construct the dendrogram based on UPGMA cluster analysis. The dendrogram indicates that the ISSR markers succeeded in distinguishing most of 20 varieties in relation to their genetic background and their geographical origin.