Marker assisted screening of breeding population of wheat segregating for stripe rust resistance using SSR markers

Abstract

Screening of F2 population from cross WH711 (susceptible parent) and PBW698 (resistant parent) against stripe rust was done on the basis of field reaction of yellow rust and 12 morphlogical traits. The genotyping of parents was done by using 80 SSR markers out of which 11 were found polymorphic. These 11 markers were used to screen 250 individuals of F2 population. Mendelian inheritance was followed by five markers viz. Xgwm631, Cfa2040, Xsps3000, Barc76 and Xgwm 130 which segregated according to expected ratio of 1:2:1 with chisquare values 5.78, 4.23, 2.75, 9.2 and 7.6 respectively. Single marker analysis showed markers Xgwm631, Cfa2040 (Yr2, Yr6), Xsps3000 (Yr10), Xgwm130 (Yr7), Xwmc407 (Lr17), Barc46, Xgwm413 (Yr15), Barc187 (Yr27) and Xgwm273 (YrH52) were linked with yellow rust locus at 0.01% of significance hence these genes may be present in the breeding population. Cfa2040 was found linked with flag leaf area, number of spikelets and Barc181 with spike weight locus. Markers on chromosomes 7A and 1B showed LOD values 6.64 and 6.86 conferring tight linkage with yellow rust locus thus can be used for MAS.