Effect of partial deoxygenated extender on different post thaw incubation periods of physico-morphological and functional attributes during cryopreservation of buffalo spermatozoa

Abstract

The study was designed to explore the effect of partial deoxygenated extender of different post thaw incubation periods on physico-morphological attributes during cryopreservation of buffalo semen. Semen samples with mass motility of ≥4+ and individual progressive motility ≥ 80 collected from murrah buffalo bulls were utilized. Immediately after collection, a portion of semen was evaluated for progressive motility, hypo-osmotic swelling response (HOS response) and acrosomal intactness and other portion was divided into two equal groups. Group I was kept as control and diluted with Egg yolk-Tris-Glycerol (EYTG) with a maximum of 60×106 sperm/mL. Group II was diluted with partially deoxygenated EYTG extender. Both the groups were subjected to equilibration and freezing. Both the group of semen samples were evaluated at pre-freeze and post-thaw stage of progressive motility, viability, HOS response and acrosomal intactness. Post-thaw motility and viability was studied at 0, 15, 30, 45 and 90 minutes of incubation. At pre-freeze and post thaw stage, significantly (p