Phytochemical screening and High-performance liquid chromatography (HPLC) profile of different extracts of Euphorbia hirta (Linn)

Abstract

The present examination plans to phytochemical screening and evaluates the total flavonoid content in the different extracts of the whole plant of Euphorbia hirta. Acetone, Chloroform, Ethylacetate, Methanol, and Hydro-alcohol (70% Methanol) were used as extraction solvents and are employed for phytochemical screening as well as HPLC analysis. Phytochemical screening of all the concentrates demonstrated and revealed the presence of flavonoids in all the extracts. HPLC was completed utilizing a segment and acetonitrile and 0.1% phosphoric corrosive in water (7:3) dissolvable framework that is portable base and recognized by wavelength 254nm and 360 nm settled for flavonoids measure. Current work explores the flavonoid (rutin) richness in the whole plant of E. hirta and future work aimed to isolate the flavonoid in the plant.