A quick method for micro-propagation of Aloe vera L. from leaf explants via callus induction

Abstract

Micropropagation or regeneration of plants depends on an efficient protocol. An efficient protocol for plant regeneration was developed in present experiment using leaves and lateral shoots explants of Aloe vera. Investigation was conducted to assess the suitability of leaf explants for callusing using different concentration of auxin (2, 4-D and NAA) cytokinin (BAP and Kinetin) in semi-solid Murashige and Skoog (MS) media. Both shoot induction and elongation were better on MS medium supplemented with 4.0 mg/L BAP+ 1.0 mg Kin highest percentage (93.33%). All cultures showed shoot regeneration in this medium with multiple shoots/callus. Root regeneration was found maximum 66.67% in half basal MS medium with combination of 3.0 mg/L 2, 4 D+ 2.0 mg NAA in all combinations of both PGRs. On the other hand, the lowest number of root per inoculated shoot was zero without PGRs. Further the regenerated plants with well-developed roots were transferred to sterilized cockpit in poly house. By this procedure in future the cost of Aloe vera production will be decreased which will lead the decrease of aloe product prices in market and the standardization of the media and PGRs combinations are very useful for future studies and micropropagation of Aloe vera L.