Determination of sulfadoxine residues in poultry meat by liquid chromatography and tandem mass spectrometry

Abstract

A study was conducted for determination of sulfadoxine residual level in poultry meat marketed in Chennai city. An LC-MS/MS analytical method has been developed and validated for the simultaneous identification, confirmation and quantitation of sulfadoxine residue. The method was validated in accordance with European commission 2002/657/EC. The target analyte from meat samples was extracted by liquid-liquid extraction with ethyl acetate and hexane. Evaporated and reconstituted samples were analysed by liquid chromatography tandem mass spectrometry (LC-MS/MS). Relative standard deviation values (RSD%) for the inter-assay variation of analyte at the three levels of fortification (5,10 and 15ppb), ranged between 4.1 to 8.2. Out of 102 poultry meat samples analysed 16.7% samples had shown detectable levels of Sulfadoxine and concentration varied from the range of 1.03 ppb to 23.8 ppb which were within maximum residue level (MRL) prescribed by European Union regulations.