Hyper-production of laccase a green catalyst by RS2 (<em>Pseudomonas stutzeri </em>CW202) through Mutagenesis

Abstract

Extracellular Laccase (EC 1.10.3.2) which is well known as a ‘Ecofriendly’ enzyme / Green catalyst has grown significantly in recent years and has wide range of industrial application from textile industries, pulp and paper industries to food industry and bioremediation processes. In this study the wild strain of Pseudomonas stutzeri CW202 was improved for enhanced Laccase production by using physical mutagen (ultraviolet rays). Mutant (M3) with hyper Laccase production was obtained after treating wild strain with ultraviolet rays on the basis of Laccase activity. Mutant M3 exhibited maximum extracellular Laccase activity after 24 hrs of incubation period at 35 °C which was assess by calculating enzyme activity at different pH and Temperature. The optimum pH for higher Laccase activity was found at pH 8 using syringaldehyde substrate which was 1098 U/L i.e. six fold increase as compared with the wild which has 213U/L. Whereas using ABTS 499U/L activity was achieved at pH 4. The optimum temperature found at 45 °C. The effectiveness of hyper Laccase producing mutant M3 indicates its possible applicability in various biotechnological and industrial processes.