Studies on efficacy of various <em>in vivo</em> and <em>in vitro</em> techniques on crossability and pod setting percentage in <em>Vigna mungo</em> x <em>V. umbellata</em> hybridization

Abstract

The present investigation was carried out with objective to study the efficacy of various in vitro and in vivo techniques for the success of interspecific hybridization between urdbean and ricebean. Interspecific crosses were attempted between three genotypes of urdbean i.e. HPBU-111, Him Mash-1 & Palampur-93 and three genotypes of ricebean i.e. PRR-1, PRR-2 & VRB-3.The study of effect of immuno- suppressants revealed that amino caproic acid at 1000 ppm was found to be the most efficient immuno- suppressant in all the three combination for achieving maximum per cent of pod set. Four minutes of UV irradiation treatment to pollen gave maximum pod setting in Palampur-93 x PRR-2 and Him Mash-1 x VRB-3. The present study revealed the presence of pre-fertilization barriers in interspecific crosses between urdbean & ricebean and were confirmed by the low frequency of pod set. Both the parents involved in interspecific hybridization shows differential genotypic response which indicates the use of more number of genotypes of urdbean and ricebean.