Isolation of quality DNA from gladiolus corms using different methods

Abstract

DNA is the principal material for numerous molecular studies in plants. Leaves are most commonly used for extraction of DNA pertaining to the ease of isolation. In this study, we have tried two different methods for isolation of quality DNA from gladiolus corms of two genotypes i.e. Psittacinus hybrid and Pink Friendship. Gladiolus corms are rich in polysaccharides and polyphenols which make extraction of DNA difficult. The two DNA isolation methods i.e. method 1 and method 2 gave different results for quality and quantity of DNA. DNA yield was found to be better in method 2 for both Psittacinus hybrid and Pink Friendship genotypes as compared to method 1. DNA bands were also found to be less smeared in method 2 with respect to method 1. Good quality DNA obtained from uncommonly used plant part like corms with a suitable method can be successfully employed for various molecular studies.