Molecular characterization and malaria transmission potential of Anopheles gambiae complex in Awka, Anambra state, Nigeria

Abstract

The molecular characterisation and malaria transmission potential of Anopheles gambiae complex mosquitoes in Awka, Anambra State were studied from June to September, 2018. Indoor and outdoor biting mosquitoes were collected by human bait and pyrethrum knockdown methods. Sporozoite index was determined with Enzyme-linked Immunosorbent Assays (ELISA). Molecular characterization of sibling species of An. gambiae complex was done with Polymerase Chain Reaction (PCR).A total of 211 An. gambiae complex mosquitoes were collected among which 80(38.0%) were outdoor biting adults while 131(62.0%) were indoor biting adults. There was a significant difference in the number of An. gambiae mosquitoes collected indoors and outdoors (P