Influence of drought stress on leaf protein pattern of maize genotypes

Abstract

For evaluation of changes in leaf protein pattern of maize, 12 maize genotypes (PDM 1409, PDM 1415, PDM 1428, PDM 1452, PDM 1465, PDM 1474, PDM 1479, PDM 1485, PDM 1488, PDM 1498, PDM 1430 and PDM 1439) were assayed under both drought stress and non-stress conditions by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE) during rabi, 2015-16 and 2016-17. Total leaf proteins were extracted and separated on 12% polyacrylamide gels using standard protocols. Protein profiling under moisture stress was also identical to the bands under irrigated condition however there is up regulation of proteins was observed. A protein of 35 kDa, 55 kDa and 155 kDa are expressed in all the genotypes. Among the genotypes these specific proteins were conspicuous in PDM 1409, PDM 1415 and PDM 1465 compared to other genotypes.