Anticoagulant activities of sulfated polysaccharides from the edible marine algae Padina tetrastromatica and Ulva fasciata: A combined in vitro and in vivo approach

Abstract

Experiment was conducted during 2013 at Agricultural Research station, Vaigaidam to identify the effect of organic priming and foliar nutrition with herbal leaf extracts of chicory (Cichorium indybus L.), clerodendron (Clerodendran inerme L.), noni (Morinda tinctoria L.) and calotropis (Calotropis procera L.) on seedling vigour and productivity potential in maize. Maize seeds were soaked in fresh leaf extracts of Cichorium indybus L.), (5, 10 and 15 %), Morinda tinctoria, Clerodendran inerme and Calotropis procera (1, 2 and 3 % ) in double the volume of herbal extract for 12 h. Seeds soaked in water (12 h) formed the control. Maize seed priming with 1 % leaf extract of Morinda tinctoria, significantly increased the germination (94 %), rate of germination (16.26) and dry matter production (2.74 g) compared to control. The electrical conductivity (0.190 dSm-1), leachate amino acids (27.52 mg), sugars (30.72 mg) and lipid peroxidation (0.110 mg) were minimum in leaf 1% extract of Morinda tinctoria but the dehydrogenase, catalase and peroxidase enzyme activity were higher (0.357, 2356 units and 0.390 mg, respectively). Combined effect of seed priming with 1% leaf extract of Morinda tinctoria and foliar nutrition spray with noni ( 2% extract) at 40 and 60 Days After Sowing increased the plant height in both kharif and rabi seasons (207 cm and 212 cm), maximum plant dryweight (266 g and 273 g), more leaf area index (11.10 and 12.03), higher crop growth rate (38.25 g and 40.80 g) and relative growth rate (25.20 mg and 26.12 mg).Tassel initiation was early (47 days and 46 days) and produced lengthier cobs (20.75 cm and 21.60 cm), more number of seeds (507 and 523), higher yield (6655 kg/ha and 6710 kg/ha) with maximum 100 seed weight (28.93 g and 28.93 g). The present study revealed that subjecting the maize to 12 h seed priming in 1 % leaf extract and foliar nutrition with 2% leaf extract of Morinda tinctoria at 40 and 60 Days after Sowing increased the seedling vigour and yield attributing factors in maize.