In vitro evaluation of inhibitory potential of Terminalia chebula (Combretaceae) fruit extracts on rat CYP enzymes

Abstract

The present study was aimed to evaluate in vitro inhibitory potential of Terminalia chebula fruit powder extracts on rat hepatic CYP enzymes. Alcoholic and hydroalcoholic extracts of Terminalia chebula fruit powder were prepared and standardized for their gallic acid content using RP-HPLC. These extracts and gallic acid were explored for their CYP3A4 enzyme inhibitory potential by conducting in vitro metabolism study. The study was based on the probe reaction of conversion of testosterone to 6hydroxytestosterone in presence and absence of extracts. Ketoconazole, a known inhibitor, was used as positive control. RP-HPLC with UV detector was used to quantify the metabolite generated in the study. Alcoholic and hydroalcoholic extract fractions and gallic acid in methanol showed inhibitory effect on rat CYP3A4 enzyme activity with IC50 values of 160, 80 and 21.21g/ml respectively. An analytical tool is designed to identify inhibitory potential of Terminalia chebula fruit powder extracts.