In vitro antibacterial activity and molecular docking studies of root extract of glycyrrhiza glabra against bovine mastitis pathogens

Abstract

The present study was conducted to assess the antibacterial activity of aqueous and methanolic root extracts of Glycyrrhiza glabra against Staphylococcus aureus, Streptococcus agalactiae and E-coli and in silico antibacterial activity against Glucoseamine-6-phosphate synthase (GlcN-6-P). The extracts prepared using soxhlet apparatus, antibacterial activity was conducted using disc diffusion method followed by MIC and MBC and the molecular docking studies was performed using ArgusLab 4.0.1. The phytochemical screening revealed the presence of alkaloids, glycosides, saponins, tannins, steroids and flavonoids. The zone of inhibition ranges from 6-14 mm and 12 -17 mm against pathogenic S. aureus, St. agalactiae and E. coli in both aqueous and methanolic extract respectively. The methanolic extract showed lower MIC and MBC as compared to acqueous extract. Among the sixteen bioactive molecules of Glycyrrhiza glabra roots screened for in silico anti-bacterial activity against Glucosamine 6 phosphate synthase enzyme, isoliquiritin showed excellent inhibitory action followed by shinflavanone and Licochalcone A respectively. In conclusion, Glycyrrhiza glabra root extract (acqueous and methanolic) has potentantimicrobial activity and can be used as raw materials for herbal therapy for bovine mastitis infection.