Effect of pesticides after 24 hours of treatment on transaminase, Alkaline phosphatase and Cholinesterase in Callosobruchus analis by filter paper impregnation method

Abstract

Enzyme estimation after 24 hours of treatment with three pesticides, the Acorus calamus (essential oil), Biosal (Neem pesticide) and Deltamethrin (Pyrethroid) was carried out (GOT, GPT, ALP and CHE) against Callosobruchus analis by Filter paper Impregnation Method. The LC50 dose was calculated of Acorus calamus, Biosal and Deltamethrin 0.9999 µl/cm2, 6.945603 µl/cm2 and 0.03472 µl/cm2. Enzyme activity after 24 hours of treatment A. calamus on C. analis GOT, GPT, ALP and CHE was 76.12%, 69.96% 21.18% and 67.97% respectively. Enzyme activity of Biosal on C. analis GOT, GPT, ALP and CHE was 75.77%, 67.84%, 16.84% and 48.64% respectively. Enzyme activity after 24 hours treatment of Deltamethrin against C. analis for GOT, GPT, ALP and CHE was 63.11%, 72.84%, 57.7% and 63.81% respectively.