Affect of naphthaleneacetic acid and gibberellic acid on in vitro proliferation and vegetative growth of potato in different combinations

Abstract

Infected planting materials and slow rate of propagation are key factors limiting the yield and quality of potato. This study evaluated the effects of brassinosteroids, a a class of steroidal phytohormones on in vitro propagation of potato in at Plant Tissue Culture Laboratory and Research Field (Garden Section) in the Department of Plant Physiology, College of Basic Science and Humanities, G.B. Pant University of Agriculture and Technology, Pantnagar, Udham Singh Nagar (Uttarakhand). The tuber yield was found to be independent of number of eyes and seed size. Tuber weight was found to be inversely proportional to percent loss of weight with time. Number of nodes proliferated, number of branches and shoot length arising from tissue culture was found highest in treatment receiving higher GA:NAA (Gibberellic acid: Naphthaleneacetic acid in 0.5:0.1 µM concentration). Higher percentage of culture survival was observed with the aforementioned ratio of GA:NAA and similar results were obtained for shoot initiation from culture.