Basics of Immunohistochemical procedure

Abstract

The field of diagnostics has emerged with some varied new techniques in recent past enabling pathologists to reach to a more conclusive diagnosis. One such technique is immunohistochemistry which in recent past has gained a lot of importance. Although histopathology has long been considered to be gold standard in diagnosis but immunohistochemistry has further facilitated pathologists to decipher the cell lineage as well. Hereby we discuss immunohistochemistry especially the procedure and principle of each step of procedure.