Effect of the hydro-alcoholic leaves extract of Paullinia pinnata (Lin.) on rats skeletal muscle regeneration after contusion injury and phytochemical analysis

Abstract

The present study aimed to investigate the muscle-protective effect of PPLE against muscle contusion injury. One hundred wistar rats were divided into five groups (n = 20): a control group, an untreated group and 3 treated groups. At day 0, all the animals except control rats were subjected to muscle contusion injury. Control and untreated groups received orally the vehicle while treated groups received the hydro-alcoholic PPLE (100, 200, and 500 mg/kg) once a day, for 14 days. The blood of five animals of each group was collected in order to evaluate the rates of LDH, CK, AST and ALT enzymes. Hydroxyproline content and histopathological analyses were investigated. Phytochemical screening was performed. Only untreated rats showed increased serum concentration and decreased hydroxyproline content. Histopathological analysis showed myotubes formation in the treated groups. Phytochemical analysis revealed the presence of tannins and flavonoids which could have positive effect on the regeneration process.