Oxidative stress induced radical scavenging, antimicrobial and cytoprotective potential on Saccharomyces cerevisiae by ethanolic extract of Melia dubia fruit pulp

Abstract

In this study antioxidant, antimicrobial and cytoprotection activity of crude ethanolic extract of Melia dubia fruit pulp was studied. Antioxidant activity was examined in different in vitro models, viz., Diphenyl picrylhydrazyl (DPPH) radical, and inhibition of reactive oxygen species (ROS), reducing power, and total phenolic content and lipid peroxidation. Cytoprotective assays performed were cell viability, Reactive oxygen species (ROS), Lipid peroxidation (TBARs), and Lactate dehydrogenase (LDH). Further the extract showed good antimicrobial activity with inhibition in growth up to 80% in different Microorganism, P. Vulgaris, S. aureus, and E. Aerogens and E. coli. From the results obtained it was observed that the DPPH radical activity showed IC 50 of 54 µg, ROS and inhibition of lipid peroxidation with an IC 50 of 100 and 80 µg respectively. The extract showed good reducing activity, which is an index of hydrogen donor. Also total phenolic content was recorded 71.2 mg/ g of the extract. Further the extract showed cytoprotective action on xenobiotic induced (CCl4) oxidative stress in Saccharomyces cerevisiae (yeast cells), from the obtained results it shows that the ethanolic extract of M.dubia is having potent free radical scavenging, antimicrobial and cytoprotection activity suggesting that the ethanolic extracts of M. dubia may contain numerous antioxidant molecules, which can effectively scavenge various ROS/free radicals under in vitro conditions. The broad range of activity of the extract suggests that M. dubia is a source of natural antioxidants, which could be considered as nutraceutical with health promoting properties in the prevention, and amelioration of degenerative diseases