An overview of purification methods for proteins

Abstract

Today proteins are being increasingly used in many industrial processes like food processing, leather, cosmetics and pharmaceutical industries as well as in organic chemistry in the area of enzymatic catalysis. Proteins are also used for various green synthesis processes. Proteins are required in the purified forms for their application in the Industry as well as in organic chemistry. In fact the purification of proteins is an important step in the protein chemistry. Strategies for protein purification include non chromatographic methods and also some modern chromatographic techniques. This paper describes some of the chromatographic and non chromatographic techniques used for protein purification. Some non chromatographic affinity based purification methods have also been proved worthy for present day industrial needs which are discussed in this paper.