Genetic diversity analysis of Deoni cattle using microsatellite markers

Abstract

The present study was undertaken for population genetic analysis of microsatellite variation in Deoni cattle using 15 microsatellite markers selected from the list suggested by FAO. All the 15 microsatellite markers were amplified and observed number of alleles ranged from five (in INRA005, INRA063, ETH225 and ILSTS006) to fourteen (in HAUT024) with total 109 alleles. The overall mean observed heterozygosity and expected heterozygosity values were 0.272 (±0.068) and 0.793 (±0.014), and ranged from 0.000 to 0.926 and 0.695 to 0.897, respectively. The overall mean PIC was 0.763. All the 15 primers used in the present study were found to be polymorphic and highly informative with the PIC values ranging from 0.643 to 0.888. The overall mean inbreeding coefficient (FIS) obtained in the present study was 0.661, suggested excess heterozygosity in the population. The I‡2 test for Hardy – Weinberg equilibrium revealed that there was significant deviation of observed allele frequencies from expected for all the loci studied. The high information of polymorphic loci throw light on the scope for maintaining variation in the population.