Recovery of bovine oocytes in respect of quality and quantity by using different techniques

Abstract

The study evaluated the best techniques (aspiration/ slicing) for the recovery of oocytes from the bovine ovary. Total 601 numbers of bovine ovaries were divided as group-I (aspiration) and group-II (Slicing). The oocytes collected from the groups were classified into 3 categories separately as Type A, B and C in respect of the morphology of cumulus cell layers tightly adhered with the zonapellucida of oocytes and cytoplasmic appearance of oocyte. The rate of recovery in aspiration technique was found to be the highest for grade A (62.27±1.60) and the least in grade C (13.98±1.41) type of oocytes; while for slicing technique, highest in grade B (51.36±2.01) and the least in grade C (18.23±1.31) type of oocytes. The rate of recovery of culturable oocytes (grade A+B) was 86.01±1.41 and 81.76±1.31, by aspiration and slicing technique, respectively. Aspiration technique is the better method for recovery of oocytes for further (in-vitro) studies.