Determination of Indaziflam and its metabolite residues in black grapes

Abstract

A simple and inexpensive method was developed using liquid - liquid extraction, together with high performance liquid chromatographic method with UV detection for determination of indaziflam and its metabolite (diamino triazine) residues in black grapes. The method was validated using fruit samples spiked with indaziflam and its metabolite (diamino triazine) at different fortification levels (0.05 and 0.5 µg/g). Average recoveries (using each concentration six replicates) ranged 84-97%, with relative standard deviations less than 3%, calibration solutions concentration in the range 0.05-5.0 µg/mL and limit of detection (LOD) and limit of quantification (LOQ) were 0.05µg/g and 0.02µg/g respectively. Finally the fruit residue samples were analyzed by HPLC.