Isolation of garcinone E from Garcinia mangostana Linn and its cytotoxic effect on sp2/0 cell lines

Abstract

Research around the world is being aimed at identifying new and effective therapeutic products to serve as a substitute for the substances that are currently being used in various industries. It is well-recognized that consumption of fruits and vegetables can reduce the incidence of degenerative diseases including cancer, heart diseases, inflammation and inflammation. Many tropical plants have biological activities with potential therapeutic applications. It has been found that Garcinone E, a xanthone derivative found in the hulls of Garcinia mangostana has potent anti-bacterial, anti-inflammatory and anti-cancer effects. This study focused on isolating Garcinone E from the pericarp of mangosteen using Soxhlet Apparatus with two solvents ethanol and ethyl acetate and further subjecting it to column chromatography and characterization using UV spectrophotometer and HPLC. The ethanolic extract gave a yield of 31.9%, showed a peak of 253nm and had a retention time of 2.78 mins which corresponds to the standard of Garcinone E. The cytotoxic effect of the isolated compound was evaluated invitro on mouse myeloma cells using Tryphan Blue dye exclusion method and MTT assay which showed that Garcinone E has potent cytotoxic effect on the Sp2/0 cell lines at 48hrs incubation time with 250µl volume of Garcinone E. Further studies could be focussed on studying the mechanism of action on the cells.