In vitro rhizogenesis of acid lime cv. PKM-1 microshoots regenerated from chemical mutagen treated explants

Abstract

In vitro regenerated microshoots of acid lime cv. PKM-1derived from EMS treated explants were induced to rooton the best rooting treatment obtained through in vitro micropropagation. The chemical mutagen EMS showed an inverse trend with respect to the rooting of the microshoots. The number of days to rooting, length and number of roots showed negative correlation with the increase in dose of the mutagen. The lowest number of days 14.65 days was observed in T1 (10 mM), followed by T2 (20 mM) i.e., 17.81. The control (T0) treatment exhibited longest root length of 4.98 cm, whereas gamma ray irradiation at T1 (10 mM) registered root length of 4.78 cm followed by T2 (20mM) with 2.64 cm of root length. Among the irradiated population, T1 treatment (10 mM) recorded more number of roots per regenerated shoot (4.78) followed by T2 (20 mM) with 2.64 roots per regenerated shoot. The lowest number of roots per shoot was observed in T4 (30 mM) with 1.34 roots. The delay in rhizogenesis may be overcome by incorporation of activated charcoal @ 0.1% in the half MS media.